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pDsRed2-C1产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VYC0694 pDsRed2-C1 Clontech 荧光蛋白报告载体 1000
900
现货

载体描述

pDsRed2-C1 encodes DsRed2, a DsRed variant that has been engineered for faster maturation

and lower non-specific aggregation. Derived from the Discosoma sp. red fluorescent protein

(drFP583), DsRed2, like its progenitor DsRed1, contains a series of silent base-pair changes

that correspond to human codon-usage preferences for high expression in mammalian cells.

In addition to these changes, DsRed2 contains six amino acid substitutions: V105A, I161T, and

S197A, which result in the more rapid appearance of red fluorescence in transfected cell lines;

and R2A, K5E, and K9T, which prevent the protein from aggregating. (DsRed2 may, however,

form the same tetrameric structure as DsRed1.) In mammalian cell cultures when DsRed2

is expressed constitutively, red-emitting cells can be detected by fluorescence microscopy

within 24 hours of transfection. Large insoluble aggregates of protein, often observed in

bacterial and mammalian cell systems expressing DsRed1, are dramatically reduced in or-

ganisms expressing DsRed2. The faster-maturing, more soluble red fluorescent protein is

also well tolerated by host cells; mammalian cell cultures transfected with DsRed2 show no

obvious signs of reduced viability—in those cell lines tested, cells expressing DsRed2 display

the same morphology (e.g., adherence, light-refraction) and growth characteristics as non-

transfected controls.

产品说明书

pDsRed2-C1 产品说明书(点击下载)

载体序列

pDsRed2-C1 载体序列(点击下载)

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