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pNTAP-B产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VPS0056 pNTAP-B Stratagene 哺乳动物表达载体 1000
900
现货

产品参数

载体用途:哺乳动物表达载体

质粒大小:4531bp

原核抗性:Kanamycin(卡那霉素)

真核筛选标记:Neomycin(新霉素)

启动子:CMV promter

标签:calmodulin-binding peptides(CBP),streptavidin-binding peptides(SBP)

表达水平:

5' 测序引物:CMV-f(5'-CGCAAATGGGCGGTAGGCGTG-3',通用测序引物,测序公司免费提供)

3' 测序引物:SV40pA-r

载体拷贝数:

载体描述

The pNTAP expression vector is used to clone a gene of interest with the SBP and CBP affinity tags adjacent to the 5´ end of the gene. The pNTAP vector is available in three different reading frames to simplify subcloning. These reading frames, designated as A, B, and C, differ only by one or two bases. Thus, each pNTAP vector has a reading frame that will allow cloning a gene of interest so that it is fused correctly with the affinity tags. The multiple cloning site (MCS) of the pNTAP vectors allows for a variety of cloning strategies, resulting in fusion of the TAP tags to the N-terminus of the protein of interest. A Kozak consensus sequence provides optimal expression of the fusion protein.
 
CBP tag:KRRWKKNFIAVSAANRFKKISSSGAL
SBP tag:MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPSGGCKLG

产品说明书

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载体序列

pNTAP-B 载体序列(点击下载)

质粒图谱

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