CC029_肿瘤细胞_细胞库_赢润生物-中国最大的基因研究资源库
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Kasumi-1, 人白血病细胞株产品信息

订购信息

货号 细胞名称 种属 价格 到货周期
CC029 Kasumi-1, 人白血病细胞株 Human 1000 现货

培养条件

RPMI-1640培养基(添加NaHCO3 2g/L),90%;优质胎牛血清,10%;双抗,1%
气相:空气 ,95%;二氧化碳,5%
温度:37℃

细胞描述

细胞生长:悬浮生长
细胞形态:成髓细胞样
细胞数量:1× 106
细胞传代:维持细胞浓度在3×105~3×106cells/mL,每周补液2~3次
细胞特性:The cell line was established from the peripheral blood of an acute myeloid leukemia (AML) patient.
This is a leukemic cell line with an 8;21 chromosome translocation. This translocation juxtaposes the AML1 with ETO (or MTG8) gene, giving rise to the fusion gene AML1-ETO (also known as AML1-MTG or RUNX1-CBF2T1), hence the cells produce chimeric AML1-ETO protein. This protein down-regulates CEBPA mRNA, protein and DNA binding activity, which is crucial for the differentiation of granulocytes.
The cells are positive for myeloperoxidase showing a morphology of myeloid maturation. In proliferation assay the cells in culture showed response to interleukin-3 (IL-3), IL-6, granulocyte colony-stimulating factor (G-CSF), and granulocytemacrophage CSF (GM-CSF), but not to IL-1 or IL-5.
Neither granulocytic nor eosinophilic maturation was observed in the in vitro liquid culture by the addition of dimethyl sulfoxide, G-CSF, or IL-5, respectively. Induction of macrophagelike cells was seen by the addition of phorbol ester. Proliferation is inhibited by 1,25S-(OH)2-16,23-diene-26-F3-10-nor D3.
细胞纯度:93%
细胞活力:87%(Viability by Trypan Blue Exclusion)
细胞检测:细胞不含有HIV-1、HBV、HCV、支 原体、细菌、酵母和真菌
细胞冻存:液氮冻存(基础培养基+10%DMSO+20%FBS)
细胞运输: 干冰运输(1 Vial)或活细胞运输(T-25 flasks)
细胞用途:只可用于科研,不可用于临床诊断和治疗

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