PC024_原代细胞_细胞库_赢润生物-中国最大的基因研究资源库
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大鼠淋巴成纤维细胞产品信息

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货号 细胞名称 种属 价格 到货周期
PC024 大鼠淋巴成纤维细胞 Rattus 3680 4-5 周

培养条件

成纤维细胞培养基(Fibroblast Medium)
气相:空气,95%;二氧化碳,5%
温度:37摄氏度

细胞描述

细胞生长:贴壁生长
细胞形态:成纤维细胞样
细胞数量:1×106
细胞传代:1:3传代;2~3天换液1次
细胞特性:Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different [1]. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen [2]. Besides, it has been reported that the growth of splenic fibroblasts has increased in lymphoproliferatived diseases [3].

RLF from ScienCell Research Laboratories are isolated from rat lymph nodes. RLF are cryopreserved at primary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. RLF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. RLF are negative mycoplasma, bacteria, yeast and fungi. RLF are guaranteed to further expand for 5 population doublings at the condition provided by ScienCell Research Laboratories.

细胞纯度:93%
细胞活力:87%(Viability by Trypan Blue Exclusion)
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌
细胞冻存:液氮冻存(基础培养基+10%DMSO+20%FBS)
细胞运输:干冰运输(1 Vial)或活细胞运输(T-25 flasks)
细胞用途:只可用于科研,不可用于临床诊断和治疗

 

 Reference

[1] Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137.
[2] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair and Regeneration (L. E. Glynn, ed.), pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[3] Domracheva, E. V., Rogova, E. M., Vorob'ev, A. L. (1984) Monolayer culture of bone marrow and splenic fibroblasts in lymphoproliferative diseases. Ter Avkh. 56:63-65.

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