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货号 种属 载体 描述 价格 周期
PR10334 Human pUC57 1500 2-3 周


Gene Promoter: human Telomerase reverse transcriptase
Specificity: Tumor
Backbone: pGL3-basic, pUC57, pcDNA3.1-EGFP, ect.




Telomerase reverse transcriptase (abbreviated to TERT, or hTERT in humans) is a catalytic subunit of the enzyme telomerase, which together with the telomerase RNA component (TERC), are the most important components of the telomerase complex. The hTERT gene, located on chromosome 5, consists of 16 exons and 15 introns spanning 35kb. The core promoter of hTERT includes 330 base pairs upstream of the translation start site (ATG), as well as 37 base pairs of exon 2 of the hTERT gene.[1] The hTERT promoter is GC-rich and lacks TATA and CAAT boxes but contains many sites for several transcription factors giving indication of a high level of regulation by multiple factors in many cellular contexts.[1] Transcription factors which can activate hTERT include many oncogenes (cancer causing genes) such as c-Myc, Sp1, HIF-1, AP2, Estrogen receptor and many more, while many cancer suppressing genes such as p53, WT1 and Menin produce factors which suppress hTERT activity .[2] Another form of up-regulation is through demethylation of histones proximal to the promoter region, imitating the low density of trimethylated histones seen in embryonic stem cells.[3] This allows for the recruitment of histone acetyltransferase (HAT) to unwind the sequence allowing for transcription of the gene.[2]

1. Cong YS, Wen J, Bacchetti S (January 1999). "The human telomerase catalytic subunit hTERT: organization of the gene and characterization of the promoter". Hum. Mol. Genet. 8 (1): 137–42.
2. Kyo S, Takakura M, Fujiwara T, Inoue M (August 2008). "Understanding and exploiting hTERT promoter regulation for diagnosis and treatment of human cancers". Cancer Sci. 99 (8): 1528–38.
3. Marion RM, Strati K, Li H, Tejera A, Schoeftner S, Ortega S, Serrano M, Blasco MA (February 2009). "Telomeres acquire embryonic stem cell characteristics in induced pluripotent stem cells". Cell Stem Cell 4 (2): 141–54.

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