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货号 种属 载体 描述 价格 周期
PR10147 Mouse pcDNA3.1-EGFP 3000 3-4 周


Gene Promoter: Smooth Muscle 22 alpha
Specificity: Ubiquitous
Backbone: pGL3-basic, pUC57, pcDNA3.1-EGFP, ect.




SM22α is a calponin-related protein that is expressed specifically in smooth muscle. The only transcription factors identified to date that control muscle gene expression in multiple muscle cell types are SRF and MEF2, which belong to the MADS-box family of transcription factors. SRF binds an A/T-rich DNA sequence, referred to as the serum response element, or CArG box. CArG boxes have been shown to play an important role in the control of several skeletal and cardiac muscle genes, such as skeletal α-actin, skeletal MLC1/3, cardiac α-actin, and cardiac α-MHC [1]. The promoter of the SM α-actin gene, which is expressed in a pattern similar to SM22α during development, also contains two CArG elements, which are required for transcriptional activity in cultured SMCs [2]. Intriguingly, there are two CArG boxes in the proximal SM22α promoter [3] that may participate in its regulation in myogenic lineages. Indeed, this proximal SM22α promoter is sufficient to direct transcription of a linked reporter gene in all three muscle lineages in transgenic mice [3].

1. Ernst H, Walsh K, Harrison CA, Rosenthal N. The myosin light chain enhancer and the skeletal actin promoter share a binding site for factors involved in muscle-specific gene expression. Mol Cell Biol. 1991;11:3735-3744.
2. Blank RS, McQuinn TC, Yin KC, Thompson MM, Takeyasu K, Schwartz RJ, Owens GK. Elements of the smooth muscle α-actin promoter required in cis for transcriptional activation in smooth muscle: evidence for cell type-specific regulation. J Biol Chem. 1992;267:984-989.
3. Li L, Miano JM, Mercer B, Olson EN. Expression of the SM22α promoter in transgenic mice provides evidence for distinct transcriptional regulatory programs in vascular and visceral smooth muscle cells. J Cell Biol. In press.

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