PR10049_启动子库_赢润生物-中国基因研究资源库
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货号 种属 载体 描述 价格 周期
PR10049 Human pUC57 4000 2-3 周

产品参数

Gene Promoter: Endothelin-1
Specificity: Ubiquitous
Backbone: pGL3-basic, pUC57, pcDNA3.1-EGFP, ect.

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产品说明

Description:
Endothelin-1 (ET-1) is a peptide synthesized by endothelial cells both in culture and in vivo. ET-1 induces contraction of smooth muscle cells and stimulates growth in a variety of mesenchymal cell types. We cloned and sequenced the human ET-1gene. The objective of the work reported here was to determine which cis-acting sequence in the ET-1 promoter region are important for transcription of the gene. Using delection mutants of the ET-1 promoter, promoter reconatitution experiments, and enhanser trap experments, we have identified a 119-base pair sequence containing two regions necessary for ET-1 promoter function. This sequence allows expression of a heterologous promoter in endothelial cells.

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