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货号 种属 载体 描述 价格 周期
PR10028 Rattus pGL3-basic 3500 3-4 周


Gene Promoter: EF1 : Elongation Factor 1
Specificity: Ubiquitous
Backbone: pDRIVE, pDRIVE5-SEAP, pGL3-basic, pUC57, pcDNA3.1-EGFP, ect.


pDRIVE-EF1α/RU5'(chimpanzee) Map 产品说明书(点击下载)

pDRIVE-EF1α/RU5'(chimpanzee) Sequence 产品说明书(点击下载)

pDRIVE-EF1α/RU5'(rat) Map 产品说明书(点击下载)

pDRIVE-EF1α/RU5'(rat) Sequence 产品说明书(点击下载)


Elongation Factor 1 prom / HTLV 5’UTR

The EF-1 alpha gene encoding elongation factor-1 alpha is an enzyme which catalyzes the GTP-dependent binding of aminoacyl-tRNA to ribosomes.
EF-1α is one of the most abundant proteins in eukaryotic cells and is expressed in almost all kinds of mammalian cells. The promoter of this ’housekeeping’ gene exhibits a strong activity, higher than viral promoters such as SV40 and RSV promoters [1] and, on the contrary to the CMV promoter, yields persistent expression of the transgene in vivo [2]. InvivoGen has cloned the EF-1α promoter region of different species, among them the chimpanzee and rat. These sequences have not been described yet. They share respectively 98.46, 47.73 and 45.05% homology with the sequence of the human EF-1α promoter.

1. Kim DW. et al. 1990. Use of human elongation factor 1 alpha promoter as a versatile and efficient expression system. Gene. 91(2):217-23.
2. Guo ZS. et al. 1996. Evaluation of promoter strength for hepatic gene expression in vivo following adenovirus-mediated gene transfer. Gene Ther. 3(9):802-10.

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