PR10001_启动子库_赢润生物-中国基因研究资源库
您好,欢迎光临赢润生物技术商城
请登录    |     免费注册     帮助中心

客服热线:0731—84317068

QQ咨询:12695920

订货邮箱:service@yrgene.com

当前位置: 首页 > 启动子库

启动子产品信息

订购信息

货号 种属 载体 描述 价格 周期
PR10001 Human pDrive 3500 3-4 周

产品参数

Gene Promoter: Beta-Actin
Specificity: Ubiquitous
Backbone: pDRIVE, pDRIVE5-SEAP, pGL3-basic, ect.

产品说明书

pDRIVE-β-actin(human) Map 产品说明书(点击下载)

pDRIVE-β-actin(human) Sequence 产品说明书(点击下载)

pDRIVE5-SEAP-β-actin(human) Map 产品说明书(点击下载)

pDRIVE5-SEAP-β-actin(human) Sequence 产品说明书(点击下载)

产品说明

Description:
Beta-actin is a highly conserved protein ubiquitously expressed in all eukaryotic cells as a component of the cytoskeleton and as a mediator of internal cell motility. A 1.2-kb fragment of the β-actin 5’ flanking region is sufficient for efficient transcription [1]. This 1.2-kb fragment contains a TATA box, a CCAAT box and two highly conserved elements. The region between +765 and +783 downstream from the cap site has enhancer activity and acts as a serum-response element in quiescent cells [2]. This 19-bp element contains a CC(A/T)6GG sequence which is found in the promoter region of many cytoskeletal and muscle-specific actin genes. The human β-actin promoter was shown to drive high levels of expression of a transgene in vitro [3]. InvivoGen provides a larger fragment that includes the 5’UTR of the β-actin gene.


1. Sugiyama H. et al. 1988. Strong transcriptional promoter in the 5' upstream region of the human beta-actin gene. Gene. 65(1):135-9.
2. Orita S. et al., 1989. Identification of a site that mediates transcriptional response of the human beta-actin gene to serum factors. Gene.75(1):13-9.
3. Muller SR. et al. 1990. Efficient transfection and expression of heterologous genes in PC12 cells. DNA Cell Biol 9(3):221-9.

立即购买 加入购物车

Copyright ©2014 长沙赢润生物技术有限公司版权所有 湘ICP备15013987号-2

技术支持