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pMAL-c4x产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VYN0215 pMAL-c4x NEB 原核表达载体 1000
900
现货

载体描述

The vector pMAL-c4X is designed to produce maltose-binding protein (MBP) fusions, where the protein of interest can be cleaved from MBP with the specific protease Factor Xa.

MBP fusions made with this vector are expressed cytoplasmically. The MBP has been engineered for tighter binding to amylose resin. 

A gene or open reading frame is inserted into a restriction site of the vector polylinker, in the same translational reading frame as the malE gene (encoding maltose-binding protein). Insertion of the DNA fragment interrupts the malE-lacZα fusion pre-existing on the vector, affording a screen for inserts on the proper indicator plates. The fusion protein thus produced can be purified by amylose affinity chromatography. The sequence coding for the four amino acids Ile-Glu-Gly-Arg is present just upstream of the XmnI site. This allows the protein of interest to be cleaved from maltose-binding protein with the specific protease Factor Xa. Fragments inserted in the XmnI site (cleaves GAAGG↓ATTTC) will produce a fusion protein that, after Factor Xa cleavage, contains no vector-derived residues on the protein of interest.

产品说明书

pMAL-c4x载体使用 产品说明书(点击下载)

载体序列

pMAL-c4X 载体序列(点击下载)

质粒图谱

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