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pSingle-tTS-shRNA产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VRC0791 pSingle-tTS-shRNA Clontech RNAi载体 1500
1400
现货

载体描述

The pSingle-tTS-shRNA vector expresses the tetracyline-controlled transcriptional suppressor (tTS), which controls expression of an shRNA sequence inserted into the shRNA cloning site. The tTS protein is a fusion of the Tet repressor protein (TetR) and the KRAB-AB silencing domain of the Kid-1 protein (SDKid-1 ), a powerful transcriptional suppressor (1, 2). In the absence of the inducer doxycycline (Dox, a tetracycline derivative), tTS binds to the tetO sequences in the modified Tet-responsive Pol III hybrid promoter (P Tight/U6 ) and blocks expression of the shRNA. As Dox is added to the culture medium, tTS dissociates from P Tight/U6 to allow Pol IIImediated transcription of the shRNA, resulting in suppression of the target gene in a highly dose-dependent manner. pSingle-tTS-shRNA also contains a ColE1 origin of replication and an ampicillin resistance gene (Amp r ) for propagation and selection in bacteria, as well as a neomycin resistance gene (Neo r ) for selection of stable transformants in mammalian cells.

 

After digesting pSingle-tTS-shRNA with HindIIIand XhoI, clone an annealed ds oligonucleotide encoding your desired shRNA sequence into the vector. Transfect the recombinant vector into cells and select stable, clonal tranformants with G418 to obtain a cell line capable of induced suppression of the shRNA's target gene.

产品说明书

pSingle-tTS-shRNA 产品说明书(点击下载)

载体序列

pSingle-tTS-shRNA 载体序列(点击下载)

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