VPI0019_载体库_赢润生物-中国最大的基因研究资源库
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pcDNA3.1(-)/myc-His C产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VPI0019 pcDNA3.1(-)/myc-His C Invitrogen 哺乳动物表达载体 1000
900
现货

产品参数

载体用途:哺乳动物表达载体

载体别名:pcDNA3.1(-)-myc-His C、pcDNA3.1/myc-His(-) C

质粒大小:5521bp

原核抗性:Ampicillin(氨苄青霉素)

真核筛选标记:Neomycin / G418

启动子:CMV promter

标签:C端带myc标签及His标签

表达水平:

5' 测序引物:CMV-f(通用测序引物,测序公司免费提供)

3' 测序引物:BGH-r(通用测序引物,测序公司免费提供)

载体拷贝数:

载体描述

最常用的哺乳动物表达载体之一。

CMV promoter强启动子调控外源基因表达,C端携带myc标签及His标签,方便进行表达检测。

载体拷贝数很高,表达量也很高。

无荧光标记。

Amp+原核筛选抗性,Neo+真核筛选抗性,可以利用G418筛选稳定细胞株。

 

pcDNA3.1/myc-His(-) A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. The vectors are supplied in three reading frames to facilitate in frame cloning, with a C-terminal peptide containing a polyhistidine metal-binding tag and the myc (c-myc) epitope. The human cytomegalovirus immediate-early (CMV) promoter provides highlevel expression in a wide range of mammalian cells. In addition, the vector will replicate episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and nonreplicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/myc-His(-)/lacZ, is the pcDNA3.1/myc-His(-) A vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. It is included for use as a positive control for transfection, expression, and detection in the cell line of choice. 

产品说明书

pcDNA3.1(-)/myc-His A&B&C 质粒图谱 产品说明书(点击下载)

pcDNA3.1(-)/myc-His A&B&C 载体使用 产品说明书(点击下载)

载体序列

pcDNA3.1(-)-myc-His C 载体序列(点击下载)

质粒图谱

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