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pcDNA3.1/V5-His C产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VPI0012 pcDNA3.1/V5-His C Invitrogen 哺乳动物表达载体 1000
900
现货

产品参数

载体用途:哺乳动物表达载体

原核抗性:Ampicillin(氨苄青霉素)

真核筛选标记:Neomycin / G418

启动子:CMV promter

标签:C端带V5标签及His标签

表达水平:

5' 测序引物:CMV-f

3' 测序引物:BGH-r

载体拷贝数:

载体描述

        pcDNA ™ 3.1/V5-His A, B, and C are 5.5 kb vectors derived from pcDNA ™ 3.1(+) and designed for high-level expression, purification, and detection of recombinant proteins in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells.

 

        The vectors contain the following elements:

1Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the V5 epitope and a polyhistidine metal-binding peptide

2Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells

3Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7)

 

        The control plasmid, pcDNA ™ 3.1/V5-His/lacZ, contains a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. pcDNA ™ 3.1/V5-His/lacZ is included for use as a positive control for transfection, expression, purification, and detection in the cell line of choice.

产品说明书

pcDNA3.1/V5-His A&B&C质粒图谱 产品说明书(点击下载)

pcDNA3.1/V5-His A&B&C载体使用 产品说明书(点击下载)

载体序列

pcDNA3.1/V5-His C 载体序列(点击下载)

质粒图谱

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