VMA0375_载体库_赢润生物-中国基因研究资源库
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pWPI产品信息

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货号 载体名称 出品公司 载体用途 价格 到货周期
VMA0375 pWPI Addgene 慢病毒载体 1000 1-2 周

载体描述

This bicistronic vector allows for simultaneous expression of a transgene and EGFP marker to facilitate tracking of transduced cells. The EGFP marker cDNA has been inserted downstream of EMCV IRES.
A popular cloning strategy is to clone into the PmeI site upstream of the EMCV IRES. You can also clone into the PacI or SwaI site.
If you want to put another fluorescent protein after the IRES, note that you need to make sure that the open reading frame of the second gene is precisely fused to 11th ATG of IRES (i.e., 11th ATG of IRES is a start of your cDNA).
Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussions on cloning strategies and protocols.
Updated sequence provided by Chang-Xin Shi, Mayo Clinic.

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