VMA0374_载体库_赢润生物-中国基因研究资源库
您好,欢迎光临赢润生物技术商城
请登录    |     免费注册     帮助中心

客服热线:0731—84317068

QQ咨询:12695920

订货邮箱:service@yrgene.com

当前位置: 首页 > 载体库

pMDLg/pRRE(pMDLg pRRE、pMDLg-pRRE)产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VMA0374 pMDLg/pRRE(pMDLg pRRE、pMDLg-pRRE) Addgene 慢病毒载体 1000
900
现货

产品参数

载体别名:pMDLg pRRE、pMDLg-pRRE

载体用途:慢病毒载体

质粒大小:8890bp

原核抗性:Ampicillin(氨苄青霉素)

启动子:CMV promter

表达水平:

5' 测序引物:CMV-f(5'-CGCAAATGGGCGGTAGGCGTG-3',通用测序引物,测序公司免费提供)

3' 测序引物:-

载体拷贝数:

扩增用宿主菌:DH5α、JM101、XL1-Blue等均可

是否已测序:已测序确认质粒正确。

备注:一般和pCMV-VSV-G、pRSV-rev配套使用包装慢病毒。

载体描述

This 3rd generation LV packaging plasmid includes gag, coding for the virion main structural proteins; pol, responsible for the retrovirus-specific enzymes; and RRE, a binding site for the Rev protein which facilitates export of the RNA from the nucleus. This plasmid does not include Rev. The third generation packaging system offers maximal biosafety but is more cumbersome, as it involves the transfection of four different plasmids in the producer cells.
Example of a 3rd generation packaging combo: pMDLg/pRRE + pRSV-Rev + pMD2.G.

Please note that most Trono lab lentivectors contain a wt 5'LTR and can be packaged only using the 2nd generation packaging system (as the wt 5'LTR requires TAT for activation).
If you wish to use the 3rd generation packaging system, you need to have a lentivector with a chimeric 5'LTR (e.g. CCL-, RRL-, etc) in which the HIV promoter is replaced with CMV or RSV, thus making it TAT-independent. The lentivectors carrying the chimeric 5'LTR can be packaged into either a 2nd or 3rd generation packaging system.

产品说明书

pMDLg pRRE 载体序列 产品说明书(点击下载)

载体序列

pMDLg-pRRE 载体序列(点击下载)

质粒图谱

立即购买 加入购物车

Copyright ©2014 长沙赢润生物技术有限公司版权所有 湘ICP备15013987号-2

技术支持