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pRS426产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VJA0558 pRS426 ATCC 酵母表达载体 1200
1000
1 周

产品参数

Size (kb): 5.726

Vector: pRS426 (phagemid)

Promoters: Promoter T7 (phi10)

Construction: pJS92, pRS306, pBluescript II SK+

Marker(s):URA3,ampR

Construct size (kb): 5.726

Features: insert detection: lacZ'

marker(s): URA3

marker(s): ampR

promoter: T3

promoter: T7 (phi10)

promoter: lac

replicon: 2 micron

replicon: f1

replicon: pMB1

MCS: KpnI...SacI

载体描述

YE-type (episomal) shuttle vector

shuttle vector
vector containing primer sites useful for sequencing
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
 
Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8; PstI--4.0, 1.7; PvuII--5.2, 0.54.
Contains the REP3 and FRT sequences necessary for high copy propagation in yeast.
In S. cerevisiae, the copy number is about 20 per haploid cell. In non-selective growth, plasmids are lost through mitotic segregation at rates in the range of 4.4 + or - 1.4% of progeny per doubling.
YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. Can be used to produce ssDNA. Contains promoters for in vitro RNA synthesis and priming sites useful for sequencing.
The order of the major features in this plasmid is: ori(f1) - lacZ - T7 promoter - MCS (KpnI-SacI) - T3 promoter - lacI - ori(pMB1) - ampR - ori(2 micron) - URA3.

产品说明书

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载体序列

pRS426 载体序列(点击下载)

质粒图谱

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