VJA0316_载体库_赢润生物-中国基因研究资源库
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YEP352产品信息

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货号 载体名称 出品公司 载体用途 价格 到货周期
VJA0316 YEP352 ATCC 酵母表达载体 1000 1-2 周

载体描述

Constructed by filling in and blunt-end ligating pUC18 linearized at NdeI, a 1.4 kb RsaI fragment of the 2 micron circle, and a 1.1 kb HindIII/SmaI fragment containing URA3. A XbaI site in 2u and a PstI site in URA3 were removed. The yeast genomic sequence is in nt 286-1383 (where the A of the ATG of lacZ' is number 1), the 2 micron sequence goes from nt 1384-2768, & pUC18 includes nt 2769-285. Restriction digests of the clone give the following sizes (kb): BamHI--5.3; XbaI--5.3; PstI--5.3; HindIII--5.3. (ATCC staff) This is a E.coli/yeast episomal shuttle vector with multiple restriction sites for cloning. (personal communication) Medium is 1227 LB plus ampicillin. NCBI gi: 295271 Hosts: E.coli RR1, S.cerevisiae, E.coli, Saccharomyces. Related vectors: pBR322, 2micron.

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