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XL2-Blue MRF’产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VDW0535 XL2-Blue MRF’ 克隆菌株 1000 现货

载体描述

XL2-Blue MRF? ultracompetent cells are high-efficiency derivatives of our XL1-Blue MRF? supercompetent cells.  Using ultracompetent cell technology, we have achieved transformation efficiencies of greater than 5 × 109 transformants/?g of pUC18 DNA, making these ultracompetent cells an ideal choice for high-efficiency cloning. The XL2-Blue MRF? (Minus Restriction) strain is deficient in all known restriction systems (McrA–, McrCB–, McrF–, Mrr–, HsdR–), preventing cleavage of cloned DNA that carries cytosine and/or adenine methylation, which is often present in eukaryotic DNA and cDNA.   XL2-Blue MRF? cells are restriction minus. The strain is endonuclease deficient (endA), greatly improving the quality of miniprep DNA, and recombination deficient (recA), helping to ensure insert stability. These cells allow blue-white screening for recombinant plasmids.

Efficiency: ≥5 x 109 transformants/?g of pUC18 DNA
Resistant to tetracycline and chloramphenicol.

产品说明书

XL2-Blue MRF’ 菌株说明 产品说明书(点击下载)

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