VDW0528_载体库_赢润生物-中国基因研究资源库
您好,欢迎光临赢润生物技术商城
请登录    |     免费注册     帮助中心

客服热线:0731—84317068

QQ咨询:12695920

订货邮箱:service@yrgene.com

当前位置: 首页 > 载体库

JM109产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VDW0528 JM109 克隆菌株 1000 现货

载体描述

Bacterial Strain JM109 is a useful host for transformation of pGEM? Vectors and for production of single-stranded DNA from M13 or phagemid vectors. The strain grows well and is transformed efficiently by a variety of methods. Because JM109 is recA– and lacks the E. coli K restriction system, undesirable restriction of cloned DNA and recombination with host chromosomal DNA are prevented. The endonuclease A– mutation leads to an improved yield and quality of isolated plasmid DNA.

JM109 is deficient in β-galactosidase activity due to deletions in both genomic and episomal copies of the lacZ gene. The deletion in the episomal (F? factor) copy of the lacZ gene (lacZΔM15) can be complemented by addition of a functional α-peptide encoded by a pGEM?-Z or pGEM?-Zf Vector. If complementation does not occur, bacterial colonies are white. To maintain the F?, JM109 should be grown on minimal (M-9) media supplemented with 1mM thiamine.

Genotype: endA1, recA1, gyrA96, thi, hsdR17 (rk, mk+), relA1, supE44, Δ( lac-proAB), [F? traD36, proAB, laqIqZΔM15].

Features - Benefits

  • Reliable: Grows well and is transformed efficiently.
  • Versatile: Useful for cloning, single-stranded DNA production, and blue/white screening.
  • High Yields of Plasmid DNA: The endonuclease A– mutation improves yield and quality of isolated plasmid DNA.

Applications

  • Blue/white color screening of pGEM?-Z and pGEM?-Zf(+/–) Vectors in the presence of X-Gal and IPTG.

产品说明书

JM109 菌株说明 产品说明书(点击下载)

载体序列

请联系YRgene技术支持索取产品载体序列。

立即购买 加入购物车

Copyright ©2014 长沙赢润生物技术有限公司版权所有 湘ICP备15013987号-2

技术支持