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Rosetta-gami B(DE3)pLysS产品信息

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货号 载体名称 出品公司 载体用途 价格 到货周期
VDN0204 Rosetta-gami B(DE3)pLysS Novagen 配套大肠杆菌 1000 现货

载体描述

Rosetta-gami B strains combine the key features of BL21 (and its Tuner? derivative), Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm. These strains are compatible with ampicillin- or spectinomycin-resistant vectors. In Rosetta-gami B(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

Genotype: F ompT hsdSB (rB mB) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pLacIRARE (CamR, KanR, TetR)

产品说明书

Rosetta-gami B(DE3)pLysS 菌株说明 产品说明书(点击下载)

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