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BL21(DE3)plysS产品信息

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货号 载体名称 出品公司 载体用途 价格 到货周期
VDN0200 BL21(DE3)plysS Novagen 配套大肠杆菌 1000 现货

载体描述

BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.

Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) pLysS (CamR)

产品说明书

BL21(DE3)plysS 菌株说明 产品说明书(点击下载)

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