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pEGFP-Actin产品信息

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货号 载体名称 出品公司 载体用途 价格 到货周期
VYC0102 pEGFP-Actin Clontech 荧光蛋白报告载体 1000 现货

产品参数


测序引物:T7 Fwd
真核筛选标记:Neomycin

载体描述

pEGFP-Actin encodes a fusion protein consisting of the red-shifted, human codon-optimized variant of green fluorescent protein (EGFP) and the gene encoding human cytoplasmic b-actin. EGFP, a derivative of the GFPmut1 variant, has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) This variant contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr, and also contains more than 190 silent base changes which correspond to human codon-usage preferences. SV40 polyadenylation signals downstream of the EGFP-Actin fusion direct proper processing of the 3' end of the EGFP mRNA. The vector backbone also contains an SV40 origin for replication in any mammalian cell line that expresses the SV40 T-antigen. A neomycin resistance cassette (Neor), consisting of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the herpes simplex virus thymidine kinase (HSV-TK) gene, allows stably transfected eukaryotic cells to be selected using G418. A bacterial promoter upstream of this cassette drives expression of the gene encoding kanamycin resistance in E. coli. The pEGFP-Actin backbone also provides a pUC origin of replication for propagation in E. coli and an f1 origin for single-stranded DNA production.

The pEGFP-Actin Vector expresses the EGFP-Actin fusion protein in mammalian cells. The protein is incorporated into growing actin filaments and allows for visualization of actin-containing subcellular structures in living and fixed cells. pEGFP-Actin can be transfected into mammalian cells using any standard transfection method. If required, stable transformants can be selected using G418.

产品说明书

pEGFP-Actin 载体说明 产品说明书(点击下载)

pEGFP-Actin 载体序列 产品说明书(点击下载)

pEGFP-Actin 限制性酶切 产品说明书(点击下载)

pEGFP-Actin 载体序列 产品说明书(点击下载)

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