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pDsRED2-ER产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VYC0093 pDsRED2-ER Clontech 荧光蛋白报告载体 1000 现货

产品参数


测序引物:T7 Fwd
真核筛选标记:Neomycin

载体描述

pDsRed2-ER is a mammalian expression vector that encodes a fusion consisting of Discosoma sp. red fluorescent protein (DsRed2); the endoplasmic reticulum (ER) targeting sequence of calreticulin, fused to the 5' end of DsRed2; and the ER retention sequence, KDEL, fused to the 3' end of DsRed2a human codon-optimized DsRed variant engineered for faster maturation and lower non-specific aggregation.
To drive expression of DsRed2, this vector contains the immediate early promoter of cytomegalovirus (PCMV IE). SV40 polyadenylation signals downstream of the DsRed2 gene direct proper processing of the 3'-end of the DsRed2 mRNA transcript. The vector also contains an SV40 origin for replication in any mammalian cell line that expresses the SV40 T-antigen, a pUC origin of replication for propagation in E. coli, and an f1 origin for single-stranded DNA production. A neomycin resistance cassetteconsisting of the SV40 early promoter (PSV40e), the neomycin/kanamycin resistance gene of Tn5 (Neor/Kanr), and polyadenylation signals from the herpes simplex virus thymidine kinase (HSV TK poly A) geneallows stably transfected eukaryotic cells to be selected using G418. A bacterial promoter (P) upstream of this cassette drives expression of the gene encoding kanamycin resistance in E. coli.

产品说明书

pDsRED2-ER 载体说明 产品说明书(点击下载)

pDsRED2-ER 载体序列 产品说明书(点击下载)

pDsRED2-ER 载体序列 产品说明书(点击下载)

pDsRED2-ER 限制性酶切 产品说明书(点击下载)

载体序列

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