VYC0082_载体库_赢润生物-中国最大的基因研究资源库
您好,欢迎光临赢润生物技术商城
请登录    |     免费注册     帮助中心

客服热线:0731—84317068

QQ咨询:12695920

订货邮箱:service@yrgene.com

当前位置: 首页 > 载体库

pEYFP-mem产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VYC0082 pEYFP-mem Clontech 荧光蛋白报告载体 1000 现货

产品参数


测序引物:T7 Fwd
真核筛选标记:Neomycin

载体描述

pEYFP-Mem encodes a fusion protein consisting of the N-terminal 20 amino acids of neuromodulin, also called GAP-43, and a yellow-green fluorescent variant of the enhanced green fluorescent protein (EGFP). The neuromodulin fragment contains a signal for posttranslational palmitoylation of cysteines 3 and 4 that targets EYFP to membranes. The EYFP gene contains four amino acid substitutions previously published as GFP-10C. The fluorescence excitation maximum of EYFP is 513 nm, and the emission maximum is 527 nm (in the yellow-green region).

In addition to the chromophore mutations, EYFP contains >190 silent mutations that create an open reading frame comprised almost entirely of preferred human codons. Furthermore, upstream sequences flanking the EYFP-Mem fusion protein have been converted to a Kozak consensus translation initiation site. These changes increase the translational efficiency of the fusion protein and consequently its expression in mammalian cells.

Expression of EYFP-Mem is driven by the immediate early promoter of CMV (PCMV IE). The vector contains an SV40 origin of replication and a neomycin resistance (Neor) gene for selection in mammalian cells. A bacterial promoter upstream of this cassette (P) expresses kanamycin resistance in E. coli. The vector backbone also provides a pUC19 origin of replication for propagation in E. coli and an f1 origin for single-stranded DNA production.

产品说明书

pEYFP-Mem 载体说明 产品说明书(点击下载)

pEYFP-Mem 载体序列 产品说明书(点击下载)

pEYFP-Mem 载体序列 产品说明书(点击下载)

pEYFP-Mem 限制性酶切 产品说明书(点击下载)

载体序列

请联系YRgene技术支持索取产品载体序列。

质粒图谱

立即购买 加入购物车

Copyright ©2014 长沙赢润生物技术有限公司版权所有 湘ICP备15013987号-2

技术支持