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RNAi-Ready pSIREN-Retro Q-ZsGreen产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VRC0347 RNAi-Ready pSIREN-Retro Q-ZsGreen Clontech RNAi载体 1000 现货

产品参数

Promoter: Human U6
Reporter: ZsGreen
Selection: prokaryotic—ampicillin
Replication: prokaryotic—Col E1 ori (low copy), viral—SV40 ori

载体描述

RNAi-Ready pSIREN-RetroQ-ZsGreen derives from our original gene silencing retroviral vector RNAi-Ready pSIREN-RetroQ. pSIREN-RetroQ-ZsGreen is a self-inactivating retroviral expression vector designed to express a small hairpin RNA (shRNA) using the human U6 promoter. This vector is provided as a linearized vector digested with BamH I and EcoR I. It is used for targeted gene silencing when an annealed DNA oligonucleotide encoding an appropriate shRNA is ligated into the vector. The pSIREN-RetroQ-ZsGreen construct can be transfected as a plasmid expression vector. For generation of active retrovirus for infecting hard-to-transfect cell lines, transfect the pSIREN-RetroQ-ZsGreen construct into a retroviral packaging cell line and collect the viral supernatant.

产品说明书

RNAi-Ready pSIREN-Retro Q-ZsGreen 载体说明 产品说明书(点击下载)

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