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pCDNA6.2/EmGFP-Bsd/V5-DEST产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VPI0578 pCDNA6.2/EmGFP-Bsd/V5-DEST Invitrogen 哺乳动物表达载体 1000 现货

产品参数

Key Function:Reporter Assays
Mammalian Selection:Blasticidin
Vector Type:pDEST,pcDNA
Cloning Method:Gateway?
Reporter:GFP (EmGFP)
Protein Tag or Fusion:V5 Epitope Tag
Constitutive or Inducible System:Constitutive
Sequencing Primer:T7 Fwd
Sequencing Primer Sequence:5'd[TAATACGACTCACTATAGGG]3'

载体描述

The Vivid Colors? pcDNA? 6.2/EmGFP-Bsd/V5-DEST vector allows you to rapidly clone your gene using Gateway? technology and simultaneously express it with Emerald Green Fluorescent Protein (EmGFP) for easy identification of transfected cells. The EmGFP is derived from well-characterized fluorescent proteins of the jellyfish Aequorea victoria (1, 2) and has been humanized for optimal mammalian expression (3) (Figure 1). If cloned with the TAG (amber) codon, your gene will be expressed in its native configuration and fully compatible with Tag-On-Demand" technology. If cloned without a stop codon, a V5-epitope tag will be fused to the carboxy-terminal end of your protein of interest. In either case, expression of EmGFP provides a bright fluorescent marker to easily identify cells co-expressing your protein. This marker allows you to focus your functional analysis on specific cells, or to sort and enrich for transfected cells in a derived population.

The Vivid Colors? pcDNA?View More 6.2/EmGFP-Bsd/V5-DEST vector provides:

  1. PGK promoter for high-level expression of EmGFP fused to the Blasticidin (bsd)-resistance marker
  2. CMV promoter for high-level expression of your protein
  3. V5-epitope tag option to fuse to the C-terminal end of your protein, if desired

pCDNA6.2/EmGFP-Bsd/V5-DEST实际上是一个双表达框载体,PGK启动子带一个EmGFP荧光标记基因,CMV启动子调控外源基因的表达。不过,外源基因插入CMV启动子后,只能用LR重组的方法实现,所以,外源基因需要先构建至Entry载体上,例如pENTR/D-TOPO等。

pCDNA6.2/EmGFP-Bsd/V5-DEST空载上,CMV启动子后有ccdB表达框,所以此载体只能在DB3.1等专用的大肠杆菌中才能复制扩增,使用此载体时,请注意配套相应的DB3.1感受态(赢润生物有售)。当外源基因成功插入,ccdB表达框将被置换掉,此时,重组质粒可以在DH5α等常规大肠杆菌中复制扩增。

产品说明书

pCDNA6.2/EmGFP-Bsd/V5-DEST 载体说明 产品说明书(点击下载)

pCDNA6.2/EmGFP-Bsd/V5-DEST 载体序列 产品说明书(点击下载)

pCDNA6.2/EmGFP-Bsd/V5-DEST MCS 产品说明书(点击下载)

pCDNA6.2/EmGFP-Bsd/V5-DEST 载体图谱 产品说明书(点击下载)

载体序列

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质粒图谱

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