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pSecTag2 HygroA产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VPI0037 pSecTag2 HygroA Invitrogen 哺乳动物表达载体 1000 1-2 周

产品参数


测序引物序列:5'd[TAATACGACTCACTATAGGG]3'
测序引物:T7 Fwd
真核筛选标记:Hygromycin

载体描述

pSecTag2 and pSecTag2/Hygro are mammalian expression vectors designed for the secretion, purification, and detection of fusion proteins. Each vector has a large multiple cloning site in three reading frames to simplify cloning in frame with the N-terminal secretion signal. The vectors (Figure 1) offer the following features:

  • Secretion signal from the V-J2-C region of the mouse Ig kappa-chain for efficient secretion of recombinant proteins (Figure 2)
  • Cytomegalovirus (CMV) promoter for high-level constitutive expression
  • C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term) Antibody
  • C-terminal c-myc epitope for detection with an Anti-myc Antibody
  • Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence to enhance mRNA stability
  • SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (e.g., COS-1, COS-7)

The pSecTag2 vectors carry the Zeocin? resistance gene for cost-effective selection in mammalian cells. Zeocin? selection can also be used in E. coli.The pSecTag2/Hygro vectors have the Hygromycin B resistance gene for selection of stable mammalian cell lines.

产品说明书

pSecTag2 HygroA 载体说明 产品说明书(点击下载)

pSecTag2 HygroA 载体序列 产品说明书(点击下载)

pSecTag2 HygroA 多聚接头 产品说明书(点击下载)

pSecTag2 HygroA 限制性酶切 产品说明书(点击下载)

载体序列

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