pSecTag2 HygroA产品信息
订购信息
| 货号 |
载体名称 |
出品公司 |
载体用途 |
价格 |
到货周期 |
| VPI0037 |
pSecTag2 HygroA |
Invitrogen |
哺乳动物表达载体
|
1000 |
1-2 周 |
产品参数
测序引物序列：5'd[TAATACGACTCACTATAGGG]3'
测序引物：T7 Fwd
真核筛选标记：Hygromycin
载体描述
pSecTag2 and pSecTag2/Hygro are mammalian expression vectors designed for the secretion, purification, and detection of fusion proteins. Each vector has a large multiple cloning site in three reading frames to simplify cloning in frame with the N-terminal secretion signal. The vectors (Figure 1) offer the following features:
- Secretion signal from the V-J2-C region of the mouse Ig kappa-chain for efficient secretion of recombinant proteins (Figure 2)
- Cytomegalovirus (CMV) promoter for high-level constitutive expression
- C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term) Antibody
- C-terminal c-myc epitope for detection with an Anti-myc Antibody
- Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence to enhance mRNA stability
- SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (e.g., COS-1, COS-7)
The pSecTag2 vectors carry the Zeocin? resistance gene for cost-effective selection in mammalian cells. Zeocin? selection can also be used in E. coli.The pSecTag2/Hygro vectors have the Hygromycin B resistance gene for selection of stable mammalian cell lines.
产品说明书
pSecTag2 HygroA 载体说明 产品说明书(点击下载)
pSecTag2 HygroA 载体序列 产品说明书(点击下载)
pSecTag2 HygroA 多聚接头 产品说明书(点击下载)
pSecTag2 HygroA 限制性酶切 产品说明书(点击下载)
载体序列
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