VPI0036_载体库_赢润生物-中国最大的基因研究资源库
您好,欢迎光临赢润生物技术商城
请登录    |     免费注册     帮助中心

客服热线:0731—84317068

QQ咨询:12695920

订货邮箱:service@yrgene.com

当前位置: 首页 > 载体库

pSecTag2 A产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VPI0036 pSecTag2 A Invitrogen 哺乳动物表达载体 1000 现货

产品参数

Mammalian Selection:Zeocin
Sequencing Primer:T7 Fwd
Sequencing Primer Sequence:5'd[TAATACGACTCACTATAGGG]3'
Tag:6X His,myc

载体描述

pSecTag2 and pSecTag2/Hygro are mammalian expression vectors designed for the secretion, purification, and detection of fusion proteins. Each vector has a large multiple cloning site in three reading frames to simplify cloning in frame with the N-terminal secretion signal. The vectors (Figure 1) offer the following features:

  • Secretion signal from the V-J2-C region of the mouse Ig kappa-chain for efficient secretion of recombinant proteins (Figure 2)
  • Cytomegalovirus (CMV) promoter for high-level constitutive expression
  • C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term) Antibody
  • C-terminal c-myc epitope for detection with an Anti-myc Antibody
  • Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence to enhance mRNA stability
  • SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (e.g., COS-1, COS-7)

The pSecTag2 vectors carry the Zeocin? resistance gene for cost-effective selection in mammalian cells. Zeocin? selection can also be used in E. coli.The pSecTag2/Hygro vectors have the Hygromycin B resistance gene for selection of stable mammalian cell lines.

产品说明书

pSecTag2 A&B&C 载体说明 产品说明书(点击下载)

载体序列

请联系YRgene技术支持索取产品载体序列。

质粒图谱

立即购买 加入购物车

Copyright ©2014 长沙赢润生物技术有限公司版权所有 湘ICP备15013987号-2

技术支持