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pEF4/V5-His A产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VPI0030 pEF4/V5-His A Invitrogen 哺乳动物表达载体 1000 现货

产品参数

Key Function:Constitutive Expression
Mammalian Selection:Zeocin
Promoter:EF-1α
Vector Type:pEF
Cloning Method:Restriction Enzyme/MCS
Protein Tag or Fusion:V5 Epitope Tag,His Tag (6x His)
Constitutive or Inducible System:Constitutive

载体描述

pEF4/V5-His A, B, and C are 5.7 kb vectors derived from pEF1/V5-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain thefollowing elements:

  1. Human elongation factor 1a-subunit promoter (hEF-1α) promoter for high-level expression across a broad range of species and cell types (Goldman et al., 1996)(Mizushima and Nagata, 1990).
  2. Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the V5 epitope and a polyhistidine metal-binding peptide.
  3. Zeocin resistance gene for selection of stable cell lines (Mulsant et al., 1988).
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen(e.g. COS7)

The control plasmid, pEF4/V5-His/lacZ is included for use as a positive control for transfection, expression, purification, and detection in the cell line of choice.

产品说明书

pEF4/V5-His A&B&C 载体图谱 产品说明书(点击下载)

pEF4/V5-His A&B&C 载体说明 产品说明书(点击下载)

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