VPI0026_载体库_赢润生物-中国最大的基因研究资源库
您好,欢迎光临赢润生物技术商城
请登录    |     免费注册     帮助中心

客服热线:0731—84317068

QQ咨询:12695920

订货邮箱:service@yrgene.com

当前位置: 首页 > 载体库

pEF1/myc-His A产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VPI0026 pEF1/myc-His A Invitrogen 哺乳动物表达载体 1000
900
现货

产品参数

载体用途:哺乳动物表达载体

载体别名:pEF1/myc-HisA、pEF1-myc-His A

质粒大小:6165bp

原核抗性:Ampicillin(氨苄青霉素)

真核筛选标记:Neomycin / G418

启动子:EF-1α promoter

标签:C端带myc标签及6xHis标签

表达水平:

5' 测序引物:T7(5'-TAATACGACTCACTATAGGG-3',通用测序引物,测序公司免费提供)

3' 测序引物:BGH-r(5'-TAGAAGGCACAGTCGAGG-3',通用测序引物,测序公司免费提供)

载体拷贝数:

载体描述

pEF1/myc-His A, B, and C are 6.2 kb vectors derived from pcDNA 3.1/myc-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain the following elements:

(1)Human elongation factor 1α subunit (hEF-1α) promoter for high-level expression across a broad range of species and cell types (Goldman et al., 1996; Mizushima and Nagata, 1990).

(2)Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the myc (c-myc) epitope and a polyhistidine metal-binding peptide.

(3)Neomycin resistance gene for selection of stable cell lines.

(4)Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g., COS7).

 

The control plasmid, pEF1/myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

产品说明书

pEF1/myc-His A&B&C质粒图谱 产品说明书(点击下载)

pEF1/myc-His A&B&C载体使用 产品说明书(点击下载)

载体序列

pEF1-myc-His A 载体序列(点击下载)

质粒图谱

立即购买 加入购物车

Copyright ©2014 长沙赢润生物技术有限公司版权所有 湘ICP备15013987号-2

技术支持