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pUB6/V5 His A产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VPI0022 pUB6/V5 His A Invitrogen 哺乳动物表达载体 1000
900
现货

产品参数

载体用途:哺乳动物表达载体

载体别名:pUB6/V5 HisA、pUB6-V5 His A

质粒大小:5463bp

原核抗性:Ampicillin(氨苄青霉素)

真核筛选标记:Blasticidin(杀稻瘟菌素)

启动子:UbC promter

标签:C端带V5标签及6xHis标签

表达水平:

5' 测序引物:T7(5'-TAATACGACTCACTATAGGG-3',通用测序引物,测序公司免费提供)

3' 测序引物:BGH-r(5'-TAGAAGGCACAGTCGAGG-3',通用测序引物,测序公司免费提供)

载体拷贝数:

 

载体描述

pUB6/V5-His A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells.

The vectors contain the following elements:

  1. Human ubiquitin C promoter (hUbC) for high-level expression across a broad range of species and cell types (Schorpp et al., 1996; Wulff et al., 1990).
  2. Three reading frames to facilitate in-frame cloning with a C-terminal peptide encoding the V5 epitope and a polyhistidine (6×His) metal-binding tag.
  3. Blasticidin resistance gene (bsd) for selection of stable cell lines.
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7).

The control plasmid, pUB6/V5-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

产品说明书

pUB6/V5-His A&B&C质粒图谱 产品说明书(点击下载)

pUB6/V5-His A&B&C载体使用 产品说明书(点击下载)

载体序列

pUB6-V5-His A 载体序列(点击下载)

质粒图谱

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