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pTracer CMV2产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VPI0016 pTracer CMV2 Invitrogen 哺乳动物表达载体 1500 现货

产品参数

Mammalian Selection:Zeocin
Marker:GFP

载体描述

这是一个双表达框载体,CMV启动子后有MCS供插入外源基因,hEF1α启动子调控Cycle 3 GFP-Zeocin? fusion的表达,可以做到既有荧光蛋白来做转染指示,且荧光基因表达和外源基因表达又不会互相干扰,而且Zeocin也可以用来做稳定性细胞筛选。另外,由于EM-7启动子的存在,Cycle 3 GFP-Zeocin? fusion在E.coli中也可以表达,可以用来做原核荧光定位。

Three pTracer? mammalian expression vectors are available that express GFP fused to the selectable marker Zeocin? . Each vector uses a different set of promoters to express the gene of interest and the Cycle 3 GFP-Zeocin? fusion.

All three pTracer? vectors have the following features:

  • Cycle 3 GFP-Zeocin? fusion for selection in mammalian cell lines
  • BGH polyA signal and transcription termination sequences to enhance mRNA stability

The pTracer?-SV40 vector offers:

  • SV40 promoter for high-level constitutive expression of your gene of interest
  • CMV promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin? fusion
  • SV40 origin for episomal replication and simple vector rescue in cell lines expressing the SV40 large T antigen

The pTracer?-CMV2 vector offers:

  • CMV promoter for high-level constitutive expression of your gene of interest
  • EF-1α promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin? fusion
  • Ampicillin resistance gene for selection in E. coli

The pTracer?-EF vectors offer:

  • EF-1α promoter for high-level constitutive expression of your gene of interest
  • CMV promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin? fusion
  • C-terminal V5 epitope tag for simple detection of recombinant fusion proteins with an Anti-V5 Antibody
  • C-terminal 6xHis tag for rapid purification on nickel-chelating resin and simple detection with an Anti-His(C-term) Antibody
  • Multiple cloning site supplied in three reading frames to simplify cloning in frame with the C-terminal tag

产品说明书

pTracer CMV2 载体说明 产品说明书(点击下载)

pTracer CMV2 载体说明 产品说明书(点击下载)

载体序列

请联系YRgene技术支持索取产品载体序列。

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