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pLPCX产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VPC0052 pLPCX Clontech 哺乳动物表达载体 1000 现货

产品参数

Key Function:Constitutive Expression
Mammalian Selection:Puromycin
Promoter:CMV
Cloning Method:Restriction Enzyme/MCS
Constitutive or Inducible System:Constitutive
Sequencing Primer:
5' primer (2844–2868):5'-AGCTCGTTTAGTGAACCGTCAGATC-3'
3' primer (3026–3001):5'-ACCTACAGGTGGGGTCTTTCATTCCC-3'

载体描述

pLPCX contains elements derived from Moloney murine leukemia virus (MoMuLV) and Moloney murine sarcoma virus(MoMuSV), and is designed for retroviral gene delivery and expression. Upon transfection into a packaging cell line, pLPCX transiently expresses, or integrates and stably expresses, a transcript containing Ψ+ signal) the puromycin resistance gene, and the gene of interest. The 5' viral LTR in this vector contains promoter/enhancer sequences that control expression of the puromycin resistance(Puro+) gene for antibiotic selection in eukaryotic cells. A gene of interest can be cloned into the multiple cloning site immediately downstream of the human cytomegalovirus (CMV) immediate early promoter. pLPCX also includes the Col E1 origin of replication and E.coli Amp+ gene for propagation and antibiotic selection in bacteria.

产品说明书

pLPCX 载体序列 产品说明书(点击下载)

pLPCX 载体说明书 产品说明书(点击下载)

载体序列

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