VPC0049_载体库_赢润生物-中国最大的基因研究资源库
您好,欢迎光临赢润生物技术商城
请登录    |     免费注册     帮助中心

客服热线:0731—84317068

QQ咨询:12695920

订货邮箱:service@yrgene.com

当前位置: 首页 > 载体库

pIRES-hyg3产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VPC0049 pIRES-hyg3 Clontech 哺乳动物表达载体 1000 现货

产品参数

Key Function:Constitutive Expression
Mammalian Selection:Neo,G418
Promoter:CMV
Cloning Method:Restriction Enzyme/MCS
Constitutive or Inducible System:Constitutive

载体描述

IRES Bicistronic Expression Vectors allow you to rapidly and efficiently select positive clones that express your target protein. They include a single cassette that expresses both your gene of interest and a selection marker or reporter gene from a single promoter, so that virtually all transfected cells expressing the selection marker also express your gene of interest. With these vectors, you can screen fewer colonies to locate clones expressing high levels of your protein. The pIRES Vectors contain the internal ribosome entry site (IRES) of the encephalomyocarditis virus (ECMV), which permits the translation of two open reading frames from one messenger RNA. Ribosomes can enter the bicistronic mRNA either at the 5' end to translate the gene of interest, or at the ECMV IRES to translate the selection marker or a reporter gene. All IRES Selection Vectors contain a partially disabled IRES which reduces the efficiency of translation initiation for the selection marker relative to that of the cloned gene, and allows preferential selection of cells expressing high levels of your protein of interest.

产品说明书

pIRES-hyg3 载体序列 产品说明书(点击下载)

pIRES-hyg3 载体说明书 产品说明书(点击下载)

载体序列

请联系YRgene技术支持索取产品载体序列。

质粒图谱

立即购买 加入购物车

Copyright ©2014 长沙赢润生物技术有限公司版权所有 湘ICP备15013987号-2

技术支持