VMA0367_载体库_赢润生物-中国基因研究资源库
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FUGW产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VMA0367 FUGW Addgene 慢病毒载体 1000
900
现货

产品参数

Gene/insert name: flap-Ub promoter-GFP-WRE
Vector backbone: HR'CS-G
Growth strain(s): Stbl3

 

载体描述

Plasmid pFUGW was constructed by inserting the following into the multicloning site of HR'CS-G: HIV-1 flap sequence PCR-amplified
from the HIV NLA4.3 genome, the human polyubiquitin promoter-C (gift of L. Thiel, Amgen), the EGFP gene, and the WRE (woodchuck
hepatitis virus posttranscriptional regulatory element) (gift of D. Trono, University of Geneva). Lentiviruses can be produced by cotransfecting the HIV-1 packaging vector Delta8.9 and the VSVG envelope glycoprotein into 293 fibroblasts.

Order of elements: CMV LTR PstI flap PacI Ubiquitin promoter SpeI HindIII PstI SalI XbaI BamHI SmaI KpnI GFP NotI EagI XbaI EcoRI EcoRV HindIII ClaI WRE ClaI SalI XhoI KpnI 3'LTR ApaI PmeI.

产品说明书

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载体序列

FUGW 载体序列(点击下载)

质粒图谱

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