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pJQ200SK产品信息

订购信息

货号 载体名称 出品公司 载体用途 价格 到货周期
VJA0527 pJQ200SK ATCC 基因置换载体(自杀型) 1500
1400
现货

产品参数

Size: 5400
Promoters: Promoter lac
Construction: P15A ori, sacB, RP4, gtmR, pBluescriptSK MCS
Marker(s):sacB,gtmR

载体描述

Suicide vector permitting gene replacement and mobilization into a wide range of Gram negative bacteria. Allows positive selection for integration. Replicates only in enterobacteria, and so functions as a suicide vector in other Gram negative hosts. When induced by media containing 5% sucrose, sacB is lethal in a wide range of Gram negative bacteria, and thus permits selection for loss of the vector. A single BglII site within the gentamicin resistance gene (gtmR) allows insertion of other resistance genes (e.g., as a BamHI cassette). The order of the major features of the plasmid is: gtmR - SstI/MCS/KpnI - P15A - traJ - oriT - sacB. 

产品说明书

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载体序列

pJQ200SK 载体序列(点击下载)

质粒图谱

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